Statistical significance was assessed by Student’s t test or the nonparametric test Kolmogorov–Smirnov. Additionally, we preliminary investigated the soluble factors present in the leukemic niche and their effect on the mesenchymal stem cells. CD34+ cell evaluations (cell cycle, self-renewal gene expression and migration capacity) were performed after 3 further days of co-culture.
After 3 days, the REH-conditioned medium was removed and freshly isolated CD34+ at a density of up to 100,000 cells/ml were added to the leukemic niche. MethodsĪ leukemic niche was established by co-culturing mesenchymal stem cells with a fresh conditioned medium obtained from a leukemic (REH) cell line. This study evaluated human HSC self-renewal potential and quiescence in an in vitro leukemic niche without leukemic cells. This interplay has also important implications for the hematopoietic stem cell (HSC) biology and function. Depending upon the nature of the study and the extent of its needs for customized programming, a charge-back arrangement may be required.Leukemic and mesenchymal stem cells interact in the leukemic microenvironment and affect each other differently.
For those studies that require a higher level of complexity and/or customization than is readily supported by REDCap, programmers experienced in clinical research data management are available through the Core Informatics Group. Many studies can be supported with REDCap alone.
#Renew flowjo license free
REDCap is available free of charge to any clinical/translational research study being conducted at Weill Cornell or any of its CTSA partner institutions.
#Renew flowjo license full
It includes a complete suite of features to support HIPAA compliance, including a full audit trail, user-based privileges, and integration with the institutional LDAP server. It provides full field validation capabilities (customized to each study), import/export (including export to common statistical packages such as SAS, SPSS and Excel), and file upload/management functions. REDCap is a PHP-based system, developed by Vanderbilt University and currently managed and updated through a national consortium, that supports the rapid setup of secure, Web-based study-specific database systems and associated forms.